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Original Experimental Research

Journal of Chinese Integrative Medicine: Volume 7 July, 2009 Number 7

DOI: 10.3736/jcim20090710

Estrogen-like effects of saikosaponin-d in mice

1.	Yong LI (Department of Gastroenterology, Shanghai Hospital of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200071, China E-mail: liyong8855@gmail.com)
2.	Peng WANG (Department of Gastroenterology, Shanghai Hospital of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200071, China )
3.	Jian-lin REN (Department of Medical Information, Shanghai Medical Information Center, Shanghai 200031, China )
4.	Dong-ying YUAN (Department of Basic Theories of Traditional Chinese Medicine, College of Basic Medical Sciences, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China )
5.	Shou-yi YIN (Department of Basic Theories of Traditional Chinese Medicine, College of Basic Medical Sciences, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China )

Objective: To investigate whether saikosaponin-d (SSd) had estrogen-like effects in mice.
Methods: Forty female BALB/c mice were randomly divided into sham-operated group, untreated group, estradiol (E₂) group, low-dose SSd (5 mg/kg) group and high-dose SSd (50 mg/kg) group, with 8 mice in each group. Except the sham-operated group, bilateral ovariectomy was performed in the mice under anesthesia with 1.25% pentobarbital (40 mg/kg). Ten days after the operation, the mice were administered with corresponding medicine by intraperitoneal injection once each day. Two weeks later, the rates of epithelial cornified cells of vagina in the mice were examined, and the body weights, uterus weights and adrenal weights were also measured. Twelve hours after the final administration, serum estradiol levels were tested by radioimmunoassay.
Results: The rates of epithelial cornified cells of vagina in mice in the high-dose SSd group and the low-dose SSd group were higher than that in the untreated group (P＜0.05), but were lower than that in the E₂ group (P＜0.05). Serum E₂ levels of mice in the SSd groups were higher than that in the untreated group (P＜0.05), but the serum E₂ level of the low-dose SSd group was lower than that in the E₂ group (P＜0.05). Uterus and adrenal indexes of mice in the SSd groups were higher than that in the untreated group (P＜0.05), however, they were lower than that in the E₂ group (P＜0.05). The body weights of the mice in the high-dose SSd group and the E₂ group were lower than that in the untreated group (P＜0.05).
Conclusion: SSd has weak estrogen-like effects in mice and may be a potential phytoestrogen.

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Li Y, Wang P, Ren JL, Yuan DY, Yin SY. J Chin Integr Med. 2009; 7(7): 657-660. Received March 24, 2009; accepted May 14, 2009; published online July 15, 2009. Indexed/abstracted in and full text link-out at PubMed. Journal title in PubMed: Zhong Xi Yi Jie He Xue Bao. Free full text (HTML and PDF) is available at www.jcimjournal.com. Forward linking and reference linking via CrossRef. DOI: 10.3736/jcim20090710

Correspondence: Yong LI, MD, Associate Professor; Tel: 021-56639310; E-mail: liyong8855@gmail.com

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