JOURNAL OF SPORTS SCIENCE & MEDICINE |
Research article |
EFFECTS OF ACUTE MODERATE AND STRENUOUS EXERCISE BOUTS ON IL-17 PRODUCTION AND INFLAMMATORY RESPONSE IN TRAINED RATS | |||||||||
Halil Duzova, Yunus Karakoc, Memet Hanifi Emre, Zumrut Yilmaz Dogan and Evren Kilinc | |||||||||
Department of Physiology, Inonu University Faculty of Medicine, 44280 Malatya, Turkey. | |||||||||
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© Journal of Sports Science and Medicine (2009) 8, 219 - 224 Search Google Scholar for Citing Articles | |||||||||
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ABSTRACT | |||||||||||||
In this study, we aimed to compare the effects of a single bout of acute moderate and strenuous running exercise on the production of interleukin-17 (IL-17), interleukin-6 (IL-6), interleukin-1 receptor antagonist (IL-1ra) and inflammatory response of skeletal muscles in regularly trained rats. Eight week old rats were trained by treadmill running 5 days per week for 13 weeks at the durations of 30 min (moderate) and 60 min (strenuous). Two days after the last training session, the animals were subjected to a single bout of moderate or strenuous exercise and serum samples were analyzed for IL-17, IL-6, IL-1ra levels and myeloperoxidase (MPO) activity of gastrocnemius muscle were determined. IL-17 level significantly increased in strenuous exercise group when compared to that of sedentary controls (p < 0.01), On the other hand, only in the moderate exercise group, there was a negative correlation between IL-6 and IL-17 levels (r = - 0.857 and p = 0.014). In conclusion, acute single bout of strenuous exercise increased IL-17 production in trained rats and, this cytokine may be involved in inflammatory process of skeletal muscles. Key words: Interleukin-17, cytokines, running, exercise, rat. |
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METHODS | |||||||||||||
Animals Training protocol Measurements Statistics |
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RESULTS | |
Initial body weights of rats in control, moderate and strenuous exercise groups and their alterations at the end of training protocol were shown in the Table 3. Increase in body weight at the end of training was found to be significant only in moderate exercise group when compared to initial values (p < 0.01). |
DISCUSSION | |||||||||||||
Our results show that a single bout of strenuous exercise increases serum IL-17 level in rats whereas a single bout of moderate exercise does not change its production in rats trained for two types of exercise model by treadmill running for 13 weeks. In our study, we also found that single bouts of both moderate and strenuous exercise elevated serum IL-6 levels in trained groups of rat, but these elevations were not statistically different from the basal levels of control group. Additionally, IL-1ra level slightly increased in both exercise groups and this result was not correlated to increase in serum IL-6 level in these exercise groups. In both moderate and strenuous exercise groups, there were no significant changes in MPO activities in gatrocnemius muscle and in blood lactate levels when compared to those of controls. |
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AUTHORS BIOGRAPHY | |
Halil DUZOVA Employment: Assoc. Prof. of Physiology, Inonu University Faculty of Medicine. Degree: M.D. Research interests: Neutrophil, exercise, cytokin. E-mail: hduzova@inonu.edu.tr hduzova@hotmail.com | |
Yunus KARAKOC Employment: Assoc. Prof. of Biophysics, Inonu University Faculty of Medicine. Degree: PhD. Research interests: Hemorheology. E-mail: ykarakoc@inonu.edu.tr | |
Memet Hanifi EMRE Employment: Prof. of Physiology, Inonu University Faculty of Medicine . Degree: PhD. Research interests: Learning, memory and antioxidant. E-mail: hemre@inonu.edu.tr | |
Zumrut Yilmaz DOGAN Employment: Research Assistant. Degree: MSc. Research interests: Expermenit animals. E-mail: byozumrut@hotmail.com | |
Evren KILINC Employment: Research Assistant. Degree: MSc. Research interests: Expermenit animals. E-mail: evren_kilinc@yahoo.com |