期刊动态
研究进展
唐山地区献血人群隐匿性乙型肝炎病毒感染分析
Analysis of occult hepatitis B virus infection among blood donors in Tangshan, China
文章发布日期： 2013年01月15日 作者： 曹晓，曹庆宝，张国强，等
下载次数:133次 点击次数:454次 【中文摘要】：目的了解唐山地区无偿献血人群隐匿性乙型肝炎感染情况。方法用ELISA法检测无偿献血者的乙型肝炎血清标志物，对于HBsAg阴性样本，进行HBV核酸检测 (NAT)，NAT阳性样本，用罗氏试剂确证HBV DNA载量。结果共检测116 741例血样，证实隐匿性乙型肝炎感染者35例，占总献血人数的0.29‰。其中97.1%隐匿性乙型肝炎感染者样本的HBV DNA滴度低于102 IU/ml。在HBV DNA 阳性人群中，抗-HBc阳性率较高，占81.5%，抗-HBs阳性或乙型肝炎病毒血清标志物全阴性也可检出HBV DNA分别占55.6%和22.9%。结论唐山地区献血人群中血清HBsAg阴性者存在一定比例的隐匿性HBV感染,其HBV病毒载量均较低, 核酸检测能够提高HBV感染的检出率。
【英文摘要】：ObjectiveTo investigate the prevalence of occult hepatitis B virus (HBV) infection (OBI) among the volunteer blood donors in Tangshan, China. MethodsEnzyme-linked immunosorbent assay was used to detect serum HBV markers in the blood donors. Nucleic acid test (NAT) was performed in the hepatitis B surface antigen (HBsAg)-negative blood samples to detect HBV DNA, and Roche reagent was used to measure the viral load of HBV DNA in NAT-positive samples. ResultsAmong the HBsAg-negative blood samples of 116 741 blood donors, 39 (0.033%) had positive results in NAT, and 35 (0.029%) were confirmed with OBI. The viral load of HBV DNA was less than 102 IU/ml in 97.1% of the blood donors with OBI. Of the blood donors with OBI, 27 (77.1%) were positive for at least one of hepatitis B surface antibody (HBsAb), hepatitis B e antigen, hepatitis B e antibody, and hepatitis B core antibody (HBcAb), and 8 (22.9%) were negative for all serum HBV markers. Of the 27 positive blood donors, 22 (81.5%) were positive for HBcAb, and 15 (55.6%) were positive for HBsAb. ConclusionOBI occurs in some HBsAg-negative blood donors in Tangshan, and the viral load of HBV DNA is low. NAT is effective in increasing the detection rate of HBV infection.
【中文关键字】：肝炎，乙型；肝炎病毒，乙型；酶联免疫吸附测定
【英文关键字】：hepatitis B; hepatitis B virus; enzyme-linked immunosorbent assay
【英文关键字】：hepatitis B; hepatitis B virus; enzyme-linked immunosorbent assay
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