To evaluate the prevalence, predisposing factors and aetiological agents of asymptomatic bacteriuria in pregnant women attending antenatal clinic at State Hospital, Ado-Ekiti, Ekiti State, Nigeria. One hundred (100) pregnant women were screened. Clean catch midstream urine samples were collected, examined microscopically and cultured. Questionnaires were administered to determine the possible predisposing factors to asymptomatic bacteriuria. 52(52%) out of the 100 women screened were positive for asymptomatic bacteriuria. 6(11.5%) out of these had mixed bacterial growth while 46(88.5%) subjects had one type of bacteria. 36% had non-significant bacterial growth. 12(12%) yielded viable growth of Candida albicans. Staphylococcus aureus was the predominant bacteria followed by Escherichia coli. Pregnant women in their third trimester had the highest prevalence of asymptomatic bacteriuria (60.3%). The age group 30-40 years had the highest prevalence (62.5%).Women with parity range of 1-2 has the highest prevalence rate of 80% while the least 27.3% was found in parity range of 0 (no previous pregnancy) Full time housewives had highest prevalence while Christian pregnant women had higher prevalent rate (66.7%) than their Muslim counterparts (58.1%) Routine screening of the urine of pregnant women for asymptomatic bacteriuria and personal hygiene was emphasized so as to reduce maternal and foetal complications associated with pregnancy.