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A simple and established method of tissue culture of human gingival fibroblasts for gingival augmentation. | Saczko | Folia Histochemica et Cytobiologica

A simple and established method of tissue culture of human gingival fibroblasts for gingival augmentation.

Jolanta Saczko, Marzena Dominiak, Julita Kulbacka, Agnieszka Chwiłkowska, Honorata Krawczykowska

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Abstract

Recent advances in tissue engineering technology suggest its application in different medical fields, including periodontology. There are some reports of new non-enzymatic methods of isolating human gingival fibroblast for short-time cultivation in vitro to be used in autologous gingival augmentation. The aim of this study was to obtain a simple and established method of culturing human gingival fibroblasts. The authors developed a recurrent method that can be successfully used in autologous gingival augmentation.

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