Original Research
Sequence and phylogenetic analysis of chicken anaemia virus obtained from backyard and commercial chickens in Nigeria : research communication
D.O. Oluwayelu, D. Todd, O.D. Olaleye
Onderstepoort J Vet Res; Vol 75, No 4 (2008), 353-357. doi: 10.4102/ojvr.v75i4.111
Submitted: 10 September 2008
Published: 10 September 2008
Abstract
This work reports the first molecular analysis study of chicken anaemia virus (CAV) in backyard chickens in Africa using molecular cloning and sequence analysis to characterize CAV strains obtained from commercial chickens and Nigerian backyard chickens. Partial VP1 gene sequences were determined for three CAVs from commercial chickens and for six CAV variants present in samples from a backyard chicken. Multiple alignment analysis revealed that the 6 % and 4 % nucleotide diversity obtained respectively for the commercial and backyard chicken strains translated to only 2 % amino acid diversity for each breed. Overall, the amino acid composition of Nigerian CAVs was found to be highly conserved. Since the partial VP1 gene sequence of two backyard chicken cloned CAV strains (NGR/Cl-8 and NGR/Cl-9) were almost identical and evolutionarily closely related to the commercial chicken strains NGR-1, and NGR-4 and NGR-5, respectively, we concluded that CAV infections had crossed the farm boundary.
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D.O. Oluwayelu,
D. Todd,
O.D. Olaleye, Metrics
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Cited-By
1. Molecular characterization of the chicken anaemia viruses isolated from broiler farms of west Azerbaijan, Iran
Hadi Nayabian, Karim Mardani
Avian Pathology vol: 42 issue: 2 first page: 108 year: 2013
doi: 10.1080/03079457.2013.766668
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