Effect of pentoxifylline on oxygen free radicals in liver as a result of ischemia-reperfusion in rats with severe hemorrhagic shock

Zhi-long GENG, Hua-mei LU, Hong CAO, Li-li NIU, Peng XU, Mu-qiang YANG, Feng ZHAO, Dong LIU

Abstract


Objective To investigate the effects of pentoxifylline(PTX) on oxygen free radicals in liver as a result of ischemia-reperfusion in rats with severe hemorrhagic shock.Method Forty-eight SD rats were randomly divided into four groups(12 each): control group(C),no resuscitation group(NR),Ringer lactate solution(LR)-treatment group(LR),and LR combined PTX(25mg/kg) group(LRPTX).The rat model of severe hemorrhagic shock was established according to Wiggers method.Blood samples were collected at Ts(before shock) and Td(before sacrifice) for determination of AST and ALT.The rats were sacrificed and the livers were obtained for the determination of the activity of SOD,the contents of MDA and iNOS,and the expression of NF-κB immediately after shock in NR group and 4 hours after resuscitation in C,LR and LRPTX group.The pathological changes in the livers were observed with light microscope and electron microscope.Results Compared with C group,the AST and ALT levels in venous blood,the contents of MDA and iNOS,and the expression of NF-κB in liver were all significantly increased in NR,LR and LRPTX groups at Td time point,while the activity of SOD significantly decreased(P < 0.05).There also showed signs of severe injury to the liver.Compared with LR group,the AST and ALT levels in venous blood,the contents of MDA and iNOS,and the expression of NF-κB were significantly decreased in LRPTX group,while the activity of SOD significantly increased(P < 0.05).Pathologic changes of injury to the liver were significantly milder.Conclusion PTX can protect liver against ischemia-reperfusion injury by decreasing iNOS production and NF-κB activation,inhibiting the generation of oxygen free radical and enhancing the activity of SOD.

Keywords


shock,hemorrhagic; reperfusion injury; reactive oxygen species

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