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STUDY OF ANTIOXIDANT POTENTIAL IN LEAVES, STEMS, NUTS OF JUGLANS REGIA L. | Ivvala Anand Shaker,Inampudi sailaja,R Swaminathan,S Saleem Basha,Kondaveeti Sureshbabu | International Journal of Bioassays


G Raja, Ivvala Anand Shaker,Inampudi sailaja,R Swaminathan,S Saleem Basha,Kondaveeti Sureshbabu


Natural antioxidants can protect the human body from free radicals and retard the progress of many chronic diseases as well as lipid oxidative rancidity in foods. The role of antioxidants has protected effect against free radical damage that may cause many diseases including cancer. Primary sources of naturally occurring antioxidants are known as whole grains, fruits, and vegetables. Several studies suggest that regular consumption of nuts, mostly walnuts, may have beneficial effects against oxidative stress mediated diseases such as cardiovascular disease and cancer. The role of antioxidants has attracted much interest with respect to their protective effect against free radical damage that may cause many diseases including cancer. Juglans regia L. (walnut) contains antioxidant compounds, which are thought to contribute to their biological properties. Polyphenols, flavonoids and flavonols concentrations and antioxidant activity of Leaves, Stems and Nuts extract of Juglans regia L. as evaluated using DPPH, ABTS, Nitric acid, hydroxyl and superoxide radical scavenging activity, lipid peroxidation and total oxidation activity were determined. The antioxidant activities of Leaves, Stems and Nuts extract of Juglans regia L. were concentration dependent in different experimental models and it was observed that free radicals were scavenged by the test compounds in all the models.


Juglans regia L., Phytochemicals, Free Radicals, Lipid Peroxidation, Antioxidants

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Khilifi S, El-Hachimi Y, Khalil A, Es Safi N, Belahyan A, Tellal R, El-Abbouyi A, In -vitro antioxidant properties of Salvia verbenaca L. hydromethanolic extract. Indian J Pharmacol 2006, 38(4)-276-280.

Yildrim A, Oktay M, Bilaloglu V, The antioxidant activity of leaves of Cydonia vulgaris. Turk J Med Sci, 2001,31: 23-27.

B Halliwell, Biochem. Soc. Symp., 1995, 61, 73.

B Halliwerll, JMC Gutteridge, CE Cross, J. Lab. Clin. Med, 1992,199, 598).

DL Madavi and DK Salunke, in eds.: SS Deshpande and DK Salunkhe, Toxicological Aspects of Food Antioxidants, Food Antioxidants, Marcel Dekker Inc. New York, 1995, pp 267.

Zhou M, Chen Y, Ouyang Q, Liu SX, Pang ZJ, Reduction of the oxidative injury to the rabbits with established atherosclerosis by protein bound polysaccharide from Coriolus vesicolor. Am. J. Chin. Med, 2000, 28: 239-249.

Almeida IF, Fernandes E, Lima JLFC, Costa PC, Bahia MF, Walnut (Juglans regia) leaf extracts are strong scavengers of prooxidant reactive species. Food Chem, 2008, 106: 1014-1020.

Bown D, Encyclopedia of Herbs and their uses. Darling Kinderley, London 1995 ISBN O-7513-020-3.

Asgary S, Parkhideh S, Solhpour A, Madani H, Mahzouni P and Rahimi P, Effect of ethanolic extract of Juglans regia L. on blood sugar in diabetes-induced rats, J. Med. Food, 2008, 11: 533-538.

Spaccarotella KJ, Kris-Etherton PM, Stone WL, Bagshaw, DM Fishell, VK West, SG, Lawrence, FR and Hartman TJ, The effect of walnut intake on factors related to prostate and vascular health in older men, Nutr. J, 2008, 7:13.

Pereira JA, Oliveira I, Sousa A, Valentão P, Andrade PB, Ferreira I, Ferreres F, Bento A, Seabra RM, Estevinho L, Walnut (Juglans regia L.) leaves: phenolic compounds, antibacterial activity and antioxidant potential of different cultivars. Food Chem. Toxicol, 2007,45, 2287–2295.

Reiter RJ, Manchester LC, Dun-Xian Tan MD, Melatonin in walnuts: influence on levels of melatonin and total antioxidant capacity of blood. Nutrition, 2005, 21: 920-924.

Amaral JS, Alves M, Seabra R, Oliveira B, Vitamin E composition of walnuts (Juglans regia L.): a 3-year comparative study of different cultivars. J. Agric. Food. Chem., 2005, 53: 5467-5472.

Li L, Tsao R, Yang R, Liu C, Zhu H, Young JC, Polyphenolic profiles and antioxidant activities of heartnut (Juglans ailanthifolia var. cordiformis) and Persian walnut (Juglans regia L.). J. Agric. Food. Chem., 2006, 54: 8033-8040.

Koksak A, Artik N, Simsek A, Gunes N, Nutrient composition of hazelnut (Corylus avellana L.) varieties cultivated in Turkey. Food. Chem., 2006, 99: 509-515.

Folin O, Ciocalteau V. On tyrosine and tryptophan determinations in proteins. J Biol Chem, 1927; 27:627-650.

Chang C, Yang M, Wen H, Estimation of total flavonoids content in propolis by two complementary colorimetric methods. J Food Drug Anala, 2002; 10:178-182.

Ermakov AI, Arasimovich VV, Smirnova-Ikonnikova MI, Yarosh NP, Lukovnikova GA. Methods of Biochemical analysis of Plants, 1972: 456.

Sreejayan N, Rao MNA, Free radicals scavenging activity by curcuminoids, Drug Res, 46, 1996, 169.

Prasanth Kumar V, Shasidhara S, Kumar MM & Sridhara BY, Effect of Luffa echinata on lipid peroxidation and free radical scavenging activity, J Pharm. Pharmacol, 52 ,2000, 891.

Re R, Pellegrini N, Protoggenete A, Pannala A, Yang M & Rice-Evans C, Antioxidant activity applying an improved ABTS radical cation assay, Free Rad Biol Med, 26, 1999, 1231- 1237.

Sreejayan N & Rao M N A, Nitric oxide scavenging activity by curcuminoids, J. Pharm Pharmacol, 47, 1997, 105.

Shirwaikar Annie, Somashekar AP, Anti-inflammatory activity and free radical scavenging studies of Aristoluchia bractoelata Lam., Indian J Pharm Sci, 65, 2003, 68.

Green LC. Wagner DA. Glogowoski J, Skipper PL, Wishnok JS, Tannenbavm SR, Analysis of nitrate and 15N in biological fluids, Anal Biochem, 126, 1982, 131.

Marcocci L, Maguire JJ, Droy-Lefaix MT, Packer L, The nitric oxide scavenging property of Ginko biloba extract EGB 761, Biochim Biophys Res Commun, 201,1994, 748.

Kunchandy E, Rao MNA. Oxygen radical scavenging activity of curcumin, Int J Pharm 1990, 58: 237 - 240.

Sanchez-Moreno C, Methods used to evaluate the free radical scavenging activity in foods and biological systems, Food Sci Tech Int, 8, 2002, 122.

Ohkawa H, Ohishi N, Yagi K, Assay for lipid peroxidation in animal tissues by thiobarbituric acid reaction, Anal Biochem, 95, 1979, 351.

Benzie IF, Strain JJ, The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power.. The FRAP assay. Anal Biochem 1996; 239:70-76.

Blois MS, Antioxidant determination by the use of stable free radicals, Nature, 26, 1958, 1199.

S Mathew, ET Abraham, In vitro antioxidant activity and scavenging effects of Cinnamomum verum leaf extract assayed by different methodologies. Food Chem. Toxicol, 2006, 44, 198-206.

Lata H, Ahuja GK, Role of free radicals in health and disease, J Physio Indian Allied Sci, 57, 2000, 124.

Ialenti A, Moncada S, Di Rosa M, Modulation of adjuvant arthritis by endogenous nitric oxide, Br J Pharmacol, 110, 1993, 701.

Ross R, The pathogenesis of atherosclerosis: a perspective for the 1990’s, Nature, 362, 1993,801.

Cotran RS, Kumar V, Collins T, in Robbin’s pathological basis of disease, 6th ed (Thomson Press (I) Ltd, Noida, India) 1999, 1.

Spencer JPE, Jenner A, Auroma OI. Intense oxidative DNA damage promoted by g - DOPA and its metabolites, implications for neurogenerative disease. FEBS Lett 1994; 353: 246- 250.

Kamalakkannan N, Stanley MPP. Effect of Aegle marmelos fruit extract on tissue antioxidants in streptozotocin diabetic rats, Ind J Exp Biol, 2003; 41: 1285-1288.

Coyle JT, Puttfarcken P, Oxidative stress, glutamate and neurodegenerative diseases, Science, 219, 1993, 1184.

Luximon-Ramma, T Bahorun, AM Soobrattee, OI Aruoma, Antioxidant activities of phenolic, proanthocyanidin and flavonoid components in Extracts of Acacia fistula. J. Agric. Food Chem, 2005, 50, 5042-5047.

M Wang, J Li, M Rangarajan, Y Shao, EJ La Voie, T Huang Cho, oxidative phenolic compounds from Sage (Salvia officinalis). J. Agric. Food Chem, 1998, 46, 4869–4873.


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