-
International Journal of Nanomedicine
- About Dovepress
Open access peer-reviewed scientific and medical journals.
- Open Access
Dove Medical Press is now a member of the Open Access Initiative
- An Author's Guide
A guide to help authors get their paper published.
- Advocacy
Support Open Access and Dove Press
- Reprints
Promotional Article Monitoring - further details
- Favored Author Program
Real benefits for authors, including fast-track processing of papers.
Antitumoral activity of L-ascorbic acid-poly-D,L-(lactide-co-glycolide) nanoparticles containing violacein
Original Research
(6484) Total Article Views
Authors:
Dorival Martins, Lucas Frungillo, Maristela C Anazzetti, et al
Published Date January 2010 Volume 2010:5 Pages 77 - 85
DOI: http://dx.doi.org/10.2147/IJN.S7833
Dorival Martins1, Lucas Frungillo2, Maristela C Anazzetti2, Patrícia S Melo3, Nelson Durán1
1Institute of Chemistry, Biological Chemistry Laboratory, Universidade Estadual de Campinas-UNICAMP, C.P. 6154, CE P 13083-970, Campinas, SP, Brazil; 2Institute of Biology, Cell Cultures and Biopharmaceutical Laboratory, Universidade Estadual de Campinas, UNICAMP, Campinas, SP, Brazil; 3Campinas Integrated Metropolitan Faculties-METROCAMP, Campinas, SP, Brazil
Abstract: It has been demonstrated that tumoral cells have a higher uptake of ascorbic acid compared to normal cells. This differential characteristic can be used as a way to improve the specificity of antitumoral compounds if combined with polymeric drug delivery systems. The aim of this study was to prepare, characterize and evaluate the antitumoral activity of poly-D,L-(lactide-co-glycolide) 50:50 loading the antitumoral compound violacein and capped with L-ascorbic acid. Nanoparticles were prepared using the nanoprecipitation method and morphologically characterized by scanning electron microscopy (SEM). The average diameter and Zeta potential were determined by photon correlation spectroscopy method (PCS), and assays were carried out to determine the content of ascorbic acid and in vitro drug release kinetics. The antitumoral activity of this system was also evaluated against HL-60 cells by tetrazolium reduction assay. Nanoparticles with size distribution between 300–400 nm and strong negative outer surface (-40 mV) were obtained by this method. Analysis of ascorbic acid content showed that this compound was mainly localized on the external surface of nanoparticles. Violacein loading efficiency was determined as 32% ± 1% and this drug was gradually released from nanoparticles at different rates depending on the composition of the release media. In addition, this system was observed to be 2 × more efficient as an antitumoral compared with free violacein.
Keywords: violacein, ascorbic acid, nanoparticles, PLGA
Post to:
Cannotea Citeulike Del.icio.us Facebook LinkedIn Twitter
Readers of this article also read:
- ASNM conference 2014
Join us at the American Society for Nanomedicine Conference in Maryland, March 28th - 30th 2014.
- Impact Factors
3.486 Drug Design
3.463 Int J Nanomedicine
2.651 Clin Interv Aging
2.073 Onco Targets Ther
2.000 Neuropsychiatr Dis
1.405 Ther Clin Risk Manag
1.333 Patient Preference - Have an opinion about one of our articles?
We encourage you to write a letter to the editor.
- Interested in being a peer-reviewer?
Click here to register.
- Pre-Submissions
Authors are welcome to send an abstract or draft manuscript to obtain a view from the Editor about the suitability of their paper. Please email here and include which journal you are interested in submitting your manuscript to. Our Editors will do a quick review of your paper and advise if they believe it is appropriate for submission to their journal.
- Formulation and evaluation of drug-loaded targeted magnetic microspheres for cancer therapy
- Fungus-mediated biological synthesis of gold nanoparticles: potential in detection of liver cancer
- Applications of gold nanoparticles in cancer nanotechnology
- Intracellular heavy metal nanoparticle storage: progressive accumulation within lymph nodes with transformation from chronic inflammation to malignancy