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Synthesis and pharmacological evaluation of azetidin-2-ones and thiazolidin-4-ones encompassing benzothiazole Gurupadayya B M, Gopal M, Padmashali B, Manohara Y N - Indian J Pharm Sci
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RESEARCH PAPER
Year : 2008  |  Volume : 70  |  Issue : 5  |  Page : 572-577
Synthesis and pharmacological evaluation of azetidin-2-ones and thiazolidin-4-ones encompassing benzothiazole


1 Department of Pharmaceutical Chemistry, J. S. S. College of Pharmacy, Mysore-570 015, India
2 Department of Studies in Biochemistry, Kuvempu University, Shivagangothri, Tholhunase, Davanagere-577 004, India
3 Sahyadri Science College, Shimoga-577 203, India
4 Department of Pharmaceutical Chemistry, National College of Pharmacy, Shimoga-577 201, India

Date of Submission 02-Mar-2007
Date of Decision 21-May-2008
Date of Acceptance 10-Sep-2008

Correspondence Address:
M Gopal
Department of Studies in Biochemistry, Kuvempu University, Shivagangothri, Tholhunase, Davanagere-577 004
India
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DOI: 10.4103/0250-474X.45393

PMID: 21394251

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   Abstract  

Various 7-chloro-6-fluoro-2-arylidenylaminobenzo(1,3)thiazole (2a-h) have been synthesized by the condensation of 7-chloro-6-fluoro-2-aminobenzo(1,3)thiazole (1) with different aromatic aldehydes. The Schiff's bases on reaction with acetyl chloride, chloroacetyl chloride and phenyl acetyl chloride yielded 1-(7-chloro-6-fluorobenzothiazol-2-yl)-3,4-substituted-aryl-azetidin-2-ones (3a-x). Similarly, cyclization of Schiff's base with thioglycolic acid furnished 3-(7-chloro-6-fluoro-benzothiazol-2-yl)-2-substituted-arylthiazolidin-4-ones (4a-h). The structures of the newly synthesized compounds have been established on the basis of their spectral data and elemental analysis. Some selected compounds were evaluated for antiinflammatory, analgesic, CNS depressant and skeletal muscle relaxant activity.


Keywords: Benzothiazole, azetidinones, thiazolidinones, pharmacological activity


How to cite this article:
Gurupadayya B M, Gopal M, Padmashali B, Manohara Y N. Synthesis and pharmacological evaluation of azetidin-2-ones and thiazolidin-4-ones encompassing benzothiazole. Indian J Pharm Sci 2008;70:572-7

How to cite this URL:
Gurupadayya B M, Gopal M, Padmashali B, Manohara Y N. Synthesis and pharmacological evaluation of azetidin-2-ones and thiazolidin-4-ones encompassing benzothiazole. Indian J Pharm Sci [serial online] 2008 [cited 2014 Mar 6];70:572-7. Available from: http://www.ijpsonline.com/text.asp?2008/70/5/572/45393


The β -lactam antibiotics are extensively used for bacterial infections. The cephalosporins [1] have withstood the onslaught of microorganisms and have come to be physician's arsenal in combating a wide range of microbial infections. Moreover various β -lactams are associated with antitumor [2] , antitubercular [3] , antiinflammatory [4] activities. Similarly, thiazolidinones have attracted considerable attention as they are also enrolled with wide range of pharmacological activities like anticonvulsant [5] , analgesic [6] and antiinflammatory [7] activities. In continuation of our studies on benzothiazole [8],[9] , we have synthesized benzothiazole moiety linked to bioactive β -lactam and thiazolidinone rings, to analyse their biological profile.

The starting material for the synthesis of desired compounds is 7-chloro-6-fluoro-2-aminobenzo(1,3)thiazole [10] (1) , which on treatment with different aromatic aldehydes in concentrated sulphuric acid yields the respective Schiff bases (2a-h). The Schiff bases were separately reacted with substituted acetyl chloride and mercaptoacetic acid produced 1-(7-chloro-6-fluorobenzothiazol-2-yl)-3,4-substituted-arylazetidin-2-ones (3a-x) and 3-(7-chloro-6-fluoro-benzothiazol-2-yl)-2-substituted-arylthiazolidin-4-ones (4a-h) respectively (Scheme 1). The newly synthesized compounds were characterized by spectroscopic data and elemental analysis and were screened for their antinflammatory, analgesic, CNS depressant and skeletal muscle relaxant activities.[Additional file 1]


   Materials and Methods   Top


Melting points were determined in open capillaries and are uncorrected. IR spectra were recorded in KBr on FTIR Shimadzu 1400S and NMR spectra were recorded on AMX-400 in CDCl 3 /DMSO- d 6 using TMS as internal standard (chemical shifts in δ ppm). Mass spectra were recorded on FT VG-7070H Mass spectrophotometer using the EI technique at 70 eV. Satisfactory elemental analyses were obtained for all the compounds and were within ±0.4% of the theoretical values. The reactions were monitored on TLC with solvents of varying polarity and the spots were located by iodine vapors. For antiinflammatory and analgesic activities, adult healthy rats of Wistar strain of either sex weighing between 150-200 g were used. For CNS depressant and skeletal muscle relaxant activity studies, adult, healthy mice of Wistar strain of either sex weighing 20-25 g were used. All animals were maintained under standard conditions and had access to pelletted animal feed and water ad libitum . The study protocols were carried out as approved by the Institutional Ethics Committee (CPCSEA Reg. No. 144).

To a mixture of 7-chloro-6-fluoro-2-aminobenzo(1,3)thiazole (1) (0.1 mol) and benzaldehyde (0.1 mol), was added two drops of concentrated sulphuric acid and the reaction mixture was refluxed in ethanol (25 ml) for 3 h. The contents were poured into cold water. The Schiff's base (2a) thus formed was filtered off and recrystallised from hot ethanol to give 7-chloro-6-fluoro-(2-hydroxy-benzylidine)-benzo(1,3)thiazole. IR (νmax ): 1650(C=N) and 3480 (Ar-OH). 1 H NMR(CDCl 3 ): 9.25 (s, 1H, -N=CH), 12.1 (s, 1H, Ar-OH), 7.0-8.0 (m, 6H, Ar-H). Mass: m/z 306 and fragment ion peaks at 289, 202, 175 and 140. Similarly, the other Schiff's bases (2b-h) were prepared.

The mixture of Schiff's base (2a) (2.90 g, 0.01 mol) and triethylamine (1.02 ml, 0.01 mol) was dissolved in dioxane (40 ml) and kept in an ice bath. To this, cold solution of acetyl chloride (0.72 ml, 0.01 mol) was added slowly at 0 0 , stirred for 10-12 h and left over night. The precipitated triethylammonium chloride was filtered off and dioxane was removed by distillation. Residue was poured into cold water; the resulting solid was dried and crystallized from ethanol to give 3a. The Schiff's bases (2b-h) were treated separately with acetyl chloride to get 3b-h. Similarly, 3i-p and 3q-x were prepared by treating 2a-h with chloroacetyl chloride and phenyl acetyl chloride separately. 3i IR (νmax ): 1650(C=O). 1 H NMR(CDCl 3 ): 3.7(d, 1H, -NCH), 3.9 (d, 1H, CHCl), 7.2-7.9 (m, 6H, Ar-H); 3q IR(νmax ): 1660 (C=O stretch) azetidinone ring, 1 H NMR(CDCl 3 ): 3.1 (d, 1H, -NCH), 3.7 (d, 1H, CH-Ar), 7.0-7.8 (m, 12H, Ar-H).

A mixture of Schiff's base 2a (2.90 g, 0.01 mol) and mercaptoaceticacid (1.19 ml, 0.01 mol) was dissolved in dioxane (20 ml). A pinch of anhydrous zinc chloride was added and then refluxed for 8 h. Separated solid was filtered, washed with sodium bicarbonate solution and then recrystallised from ethanol. Similarly, the other compounds (4b-h) were prepared. 4a IR (νmax ): 1660 (C=O) thiazolidine ring. [1] H NMR(CDCl 3 ): 3.8 (s, 2H, CH 2 ), 3.6 (s, 1H, -NCH), 7.2-7.6 (m, 7H, Ar-H). Mass: m/z 364 and fragment ions peak at 289, 202, 175, 101, 81 and 69. Physical data of the compounds is given in [Table 1].

Antiinflammatory activity:

Antiinflammatory activity of some selected compounds was evaluated using carrageenan induced rat hind paw oedema method [11] . The animals were divided into control, standard and test groups, each consisting of six animals. The first group was treated with Tween-80 (1%) suspension which served as control, second group was administered with a dose of 20 mg/kg suspension of diclofenac sodium intraperitoneally which served as standard and other groups were treated with 30 mg/kg of suspension of test compounds in Tween-80. After 30 min, the rats were injected with 0.1 ml of carrageenan (1% w/v) to the sub plantar region of left paw of the rats. The volume of paw was measured using potassium permanganate solution displacement technique with the help of plethysmograph both in control and animals treated with standard and test compounds at 0, 1, 2 and 3 h after injection of carrageenan. The percentage inhibition of oedema was calculated by using formula, percent inhibition = (1-Vt/Vc)×100, where Vt is the mean paw volume of the test drug, Vc is the mean paw volume of the control. The results are recorded in [Table 2].

Analgesic activity:

The animals were divided into four groups of six animals each. The animals, which showed reaction time of 2-3 s, were selected for experiment and analgesic activity of some selected compounds was studied by tail flick method [12] . The tail received radiant heat from a wire, which is heated by passing a current of 6 mA. The time taken for the withdrawal of tail was recorded before the administration of the compounds and for 30, 60, 90 and 120 min after administration of compounds. The cut off time for determination of latent period was taken as 40 s to avoid the injury to the skin and based on our pilot studies. One group served as a standard (pentazocine hydrochloride) with dose of 10 mg/kg body weight and another group served as control (1% Tween-80) and rest of the groups used for the test drugs. The test compounds and pentazocine hydrochloride were suspended in 1% Tween-80 which was used as vehicle for the control group. The tested compounds were administered at the dose of 30 mg/kg in the form of suspension and administered intraperitoneally. The results of analgesic activity are shown in [Table 3].

CNS depressant activity:

The CNS depressant activity [13] of the compounds was studied on mice using actophotometer. Animals of either sex weighing 25-30 g were divided into groups of six each. The actophotometer counts for 10 min were recorded by placing the animals in the actophotometer, which gives the initial reading. The compounds were administered intraperitonially in the form of suspension prepared in 1% Tween-80 at a dose of 30 mg/kg body weight. Each group served as its own control. Diazepam was administered as standard to one of the test group at a dose of 5 mg/kg body weight. After 30 min and 60 min of administration of test compound, the actophotometer counts were noted for 10 min. Decrease in the number of counts for each group was recorded and finally, the percentage CNS depressant activity was determined. The results of CNS depressant activity are shown in [Table 4].

Muscle relaxant activity:

Some of the synthesized compounds were also tested for muscle relaxant activity [14] (muscle grip strength) in mice using rotorod apparatus. The animals were trained to remain on the rotating rod (moving at speed of 25 rpm) for 5 min. The animals which were able to remain on the rotating rod for 5 min and above were selected for study and were divided into groups of six each. The compounds were administered intraperitonially at a dose of 30 mg/kg body weight in the form of suspension in 1% Tween-80 while one group received diazepam as a standard drug at a dose of 4 mg/kg body weight. After 30 min, the mice were placed again on the rotating rod and animal's fall off time was recorded. The percentage muscle relaxant activity was calculated and the results are shown in [Table 5].


   Results and Discussion   Top


Two new series of compounds namely substituted azetidinones (3a-x) and thiazolidinones (4a-h) possessing fluoro-benzothiazoles have been synthesized by using experimental protocol as shown in Scheme 1. All the derivatives were supported by spectral data. The IR, 1 H-NMR and Mass spectra are in agreement with the proposed structures.

The result of the antiinflammatory activity summarized in [Table 2] indicated that 3-chloro-azetidinones showed better activity among the tested compounds. Further the presence of ortho substituted aryl ring at 4 th position of azetidinones moiety increased the anti inflammatory activity. Substitution of chloro group with phenyl ring at 3 rd position of the azetidinone generally causes decrease in the activity. Among the thiazolidinones, 2-nitro phenyl attached to 5 th position of thiazolidinone ring showed maximum activity.

The analgesic activity summarized in [Table 3], indicated that 3a and 3c showed maximum analgesic activity. The compounds 4a and 4c exhibited higher analgesic activity than the standard pentazocine at 15, 30 and 45 min interval. However the activity decreases after 60 min suggesting shorter duration of activity for the same.

The CNS depressant activity [Table 4] reveals that 2-hydroxyl phenyl substituted azetidinone (3c) and thiazolidinone (4c) showed better depressant activity. However activity decreases after 60 min indicating that the molecules to be short acting CNS depressants. The skeletal muscle relaxant activity [Table 5] indicated that the thiazolidinones (4a, 4c and 4f) and azetidinone (3a) showed significant muscle relaxant activity.


   Acknowledgements   Top


Authors are thankful to the Professor and Chairman, Department of Studies in Biochemistry, for providing laboratory facilities and the Board of members, National Education Society and National College of Pharmacy, Shimoga, for providing facilities to conduct pharmacological activities.

 
   References   Top

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2. Veinberg G, Shestakova I, Vorona M, Konepe I, Lukevics E. Synthesis of Antitumour 6-Alkulidine Penicillanate Sulfones and Related 3-Alkylidine-2-Azetidinones. Bioorganic Med Chem Lett 2004;14:147-50.  Back to cited text no. 2    
3. Udupi RH, Mayur YC, Bhatt AR. Synthesis and Biological Activity of Certain Azetidin-2-ones. Indian J Heterocycl Chem 1997;6:281-6.  Back to cited text no. 3    
4. Bansal E, Kumar A, Verma RS, Saxena KK, Srivastava VK. Synthesis and Antiinflammatory Activity of Substituted Azetidinylthiazolyl/oxazolyl-benzidines. Indian J Heterocycl Chem 2000;9:301-06.  Back to cited text no. 4    
5. Ragab FA, Eid NM, el-Tahab HA. Synthesis and Anticonvulsant Activity of New Thiazolidinones and Thixoimidazolidinone Derivatives Derived from Furochromones. Pharmazie 1997;52:926-9.   Back to cited text no. 5    
6. Fahmy HH, El-Eraky W. Synthesis and Evaluation of Analgesic and Antiinflammatory Activities of O-Substituted Salicylamides. Arch Pharm Res 2001;24:171-9.  Back to cited text no. 6  [PUBMED]  
7. Previtera T, Vigorita MG, Fenech G, Zappala C, Giordano A, Monforte MT, et al. 3,3'-Bi-[1,3-thiazolidine-4-one] System. VII. Synthesis and SARS of Some 2-Heteroaryl Derivatives with Antiinflammatory and Related Activities. Farmaco 1994;49:33-40.  Back to cited text no. 7    
8. Gurupadayya BM, Manohara YN, Gopal M. Synthesis and Biological Activities of 2 H -3-(6'-fluoro-7'-substituted-2'-aminobenzothiazolyl)-3,4-dihydro-1,3-Benzoxazines. Indian J Heterocycl Chem 2006;15:113-6.  Back to cited text no. 8    
9. Gurupadayya BM, Gopal M, Padmashali B, Vaidya VP. Synthesis and Bological Activities of Fuorobenzothiazoles. Indian J Heterocyclic Chem 2006;15:169-72.  Back to cited text no. 9    
10. Gurupadaiah BM, Jayachandran E, Shivakumar B, Nagappa AN, Nargund LVG. Synthesis of Benzothiazole Sulphonamides and Their Antibacterial Activity. Indian J Heterocycl Chem 1998;7:213-6.  Back to cited text no. 10    
11. Kasture SB, Veena Kasture S, Phate RP, Pal SC. Pharmacological Screening of Parkia Biglandulosa Leaves-II. Indian Drugs 1999;36:588-92.  Back to cited text no. 11    
12. Kaskhediker SG, Trivedi P, Abolare GT, Goud RS, Chaturvedi SC. Synthesis and Analgesic activity of Some N-Mannich Bases of Aanthranilamide. Indian Drugs 1991;28:232-5.  Back to cited text no. 12    
13. Kulkarni SK. Hand Book of Experimental Pharmacology. 3rd ed. New Delhi: Vallabh Prakashana; 1999.  Back to cited text no. 13    
14. Puratchikody A, Gopalkrishnan S, Nallu M. Synthesis and Pharmacological Evaluation of Some Potent-2-(4-Substituted Phenyl)-1 H -Imidazoles. Indian J Pharma 2005;67:725-31.  Back to cited text no. 14    



 
 
    Tables

  [Table 1], [Table 2], [Table 3], [Table 4], [Table 5]

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