PRODUKSI SIKLODEKSTRIN DARI PATI GARUT MENGGUNAKAN BERBAGAI KOMBINASI ENZIM [Production of Cyclodextrin from Arrowroot Starch by Using the Combination Enzymes]
Abstract
PRODUKSI SIKLODEKSTRIN DARI PATI GARUT MENGGUNAKAN
BERBAGAI KOMBINASI ENZIM
[Production of Cyclodextrin from Arrowroot Starch by Using the Combination Enzymes]
Erliza Noor* dan Liesbetini Hartoto
Departemen Teknologi Industri Pertanian, Fakultas Teknologi Pertanian, Institut Pertanian Bogor
Diterima 26 November 2009 / Disetujui 27 Oktober 2011
ABSTRACT
The research was aimed to produce cyclodextrin from arrowroot starch using a combination of starch hydrolysis enzymes (a-amylase, b-amylase, pullulanase and glucoamylase). Cyclization process to form cyclodextrin was obtained using Cyclodextrin-glycosyl-transferase (CGTase). a-amylase showed better performance and faster to hydrolyze arrowroot starch as compared to that of β-amylase. Glucoamylase also was gave better result than pullulanase to break the α-1,6-glycosidic chain. Combination of α-amylase and glucoamylase were more efficient for hydrolysis process and cyclodextrin production.
Key words: arrowroot starch, cyclodextrin, cyclodextrin-glycosyl-transferase, a-amylase, b-amylase, pullulanase, glucoamylase