Isolation of chlorophyll a from spinach leaves
Abstract
An efficient method for separating chlorophyll a from spinach leaves by column chromatography and solvent extraction techniques has been developed. The purity and identity of the chlorophyll a have been confirmed by UV-Vis, IR and mass spectrometry. Yields from 100 g of freeze-dried spinach were 23 – 24 mg of chlorophyll a.
KEY WORDS: Chlorophyll a, Spinach leaves, Separation, Column chromatography, Solvent extraction, Pheophytin a
Bull. Chem. Soc. Ethiop. 2008, 22(2), 301-304.
DOI: http://dx.doi.org/10.4314%2Fbcse.v22i2.61302
http://dx.doi.org/10.4314/bcse.v22i2.61302
Bulletin of the Chemical Society of Ethiopia. ISSN: 1011-3924