On treatment with sublethal dose of triazophos exposure i.e., 24, 48, 72 and 96 h on protein metabolism of fish Channa punctatus were observed. The harmful toxic effect of triazophos, is an Organo Phosphorus (OP), is investigated by measuring key enzymes in protein metabolism, Viz. catalases, Super Oxide Dismutase (SOD) and Xanthine Oxidase (XOD) and these antioxidant enzymes were altered. In the present investigations the activity of catalases reduced under toxicity because that triazophos initiates the enhancement of super oxide radicals. The inhibition of SOD activity in the fish reveals the impairment of antioxidant defense mechanism and also reduction in molecular oxygen and normal metabolism step. The decreased activity of XOD leads to increase in cellular damage through photolytic activity or non availability of iron under stress condition.