Molecular Profiling of Interspecific Lowland Rice Progenies Resulting from Crosses between TOG5681 and TOG5674 (Oryza glaberrima) and IR64 (Oryza sativa)

Abstract

Two Outstanding Oryza glaberrima (2n = 24, AA) varieties TOG5681 and TOG5674 were used as male donor parents with IR64, the high-yielding improved Asian rice variety used as recurrent female parent by the International Rice Research Institute (IRRI) to develop 18 BC₃F₁ interspecific lowland rice progenies. The proportion of parental genomic contribution and the extent of genetic differences among these lines were assessing using 36 microsatellites markers. The average genomic contribution of the donor TOG5681 and that of the recurrent IR64 within their 12 interspecific lines derived from IR64xTOG5681 cross were estimated to 13.2% and 79.8% respectively. Using 33 out of the 36 SSR markers, the average genome introgression rate of TOG5674 and that of IR64 within their 6 progenies were estimated to 8.7% and 85.5% respectively. In addition, heterozygosity and non-parental alleles were also identified. Clustering analysis technique using NTSYS classified the progenies into six groups and group five is closely related to IR 64.