Background: Foods are enriched with variety of chemical elements. Some of these elements are necessary for human health. These elements enter in liquid, the cells and other organs, certain amount of these elements are essential for body organs to work properly.

Objectives: This research was conducted to compare the level of lead and cadmium contamination in garlic species planted in Ramhormoz and Rasht and discuss about the source of this contaminations.

Materials and Methods: Twenty composite samples were collected from each province, Khozestan (Ramhormoz) and Gilan (Rasht). Each sample was burned according to instruction reported in AOAC. Then the white residue was dissolved in diluted nitric acid then the Pb and Cd contents were determined using graphite furnace atomic absorption spectrometer at 217 and 228.8 nm, respectively. No Pb level was detected in garlic cultured in both cities. The mean concentration of cadmium was 0.40 and 0.58 mg/kg in garlics planted in Ramhormoz and Rasht, respectively.

Results: Statistical analysis showed significant difference between mean concentration of cadmium in Rasht garlic and the permitted value (P < 0.001). The cadmium level in Rasht garlic is much higher than the standard level.

Conclusions: In order to test the reliability of the data obtained using this method, randomly selected samples were spiked with Pb and Cd standard solutions. The cadmium and lead recovery were reported 93.93 and 90.18 percent.

Foods are enriched with variety of chemical elements. Some of these elements are necessary for human body. These elements are enter as liquid, cells and other body organs and perhaps the existence of certain amount of these elements are necessary for body organs to work properly. Lead and Cadmium are two unnecessary ele­ments that accumulate the living organs for which these elements are toxic (1). Lead accumulates in the bodyand interferes in Vitamin D and Calcium metabolites; it is a neurotoxin whichcauses behavioral abnormalities (1). Cadmium ion are easily absorbed by vegetables and ani­mal food distributed in kidney and liver consequents to many health problmes (2-4) it was shown that rice wheat, oyster, mussels and the kidney cortex of animals con­tain the highest amount of cadmium. (5, 6) Vegetables, especially leaf vegtables may contain elevated lead level when grown near sources of lead (7-11). vegetables grown in highly contaminated soils would obviously contain a high cadmium level. Hence determination of lead and cadmium contents of vegetables and complementary pharmaceutical is an important issue which attracts sci­entist's attentions all over the world (12-16). Even different reliable methods have been reported in the literature (17-19). Garlic is both a spicy food additive and herbal medi­cine. In Iran especially in North region, high amount of raw and cooked garlic is used daily. In result, the expo­sure incidence of these region population that leads to health hazards are significantly high. a periodical moni­tor of the extent of heavy metals contaminations in gar­lic, or further vegetables is mandatory.

This research was conducted to compare the level of lead and cadmium contamination in garlic species planted in Ramhormoz and Rasht and discus about the sources of these contaminations.

All chemicals used in the present study, excluding Pb and Cd standard solutions, were analytical reagent grade and purchased from Merck, Germany. Stock solutions of 1000 μg.mL^-1 of Pb²⁺ and Cd²⁺ were purchased from Chem Lab N.V., Belgium. GF/B filter papers were used for filtration purposes. Doubly distilled water was used in this study.

100 μg.mL^-1 of lead and cadmium solution: 10 mL of lead and cadmium stock solutions were transferred to two seprate 100 mL volumetric flasks and disolved in distilled water. Working standard solutions identified in the instrument cook book and AOAC methods were prepared by successive dilutions of these intermediate solutions.

3.4. Graphite Furnace Atomic Absorption Determination

20 microliter of sample solution was transferred to the graphite tube then 5µl %1 orthophosphoric acid was added as modifier. Finally the program was run and the data was reported (20). The Pb and Cd analyzing Furnace programs are shown in (Table1).

Table 1 Temperature Programs Set on the Graphite Furnace AAS for the Determination of Pb and Cd in Garlic Samples

3.5. Recovery

In order To determine the analysis accuracy, exact amounts of standards were added separately to two randomly selected samples prepared by dry ashing and wet digestion methods. The recovered standards were calculated from the difference and reported in (Table 2).

Table 2 Comparisons of Wet Digestion and Dry Ashing Reliability during Sample Preparation

In order to select more reliable and easy digestion methods for preparing garlic samples two standard prep­aration methods (wet digestion and dry ashing) (20, 21) were selected, the accuracy of the data obtained by these two methods were evaluated by spiking certain amounts of standards in both prepared garlic samples. Table 2 showed that the extent of matrix effects were higher in wet digestion according to AOAC suggestion, dry ashing was chosen for the present study analyzes (20).

The analysis results of 20 composite samples collected from the two cultivated areas shown in (Table 3)and con­firmed the availability of No Pb. This is consistent with the results obtained from root vegetables by other re­searchers (13). the same results were obtained from on­ion samples in our research group (22). Formation of insoluble Pb compounds with some anions especially phosphate, immobilizes the metal in soil and water a (23, 24) and reduces lead bioavailability consequently vege­tables mostly arecontaminated with atmosphere Pb. The Mean Cd contents of cultivated garlics in both provinces were higher than standard level of 0.2 mg/kg, reported by Codex (25) as it is graphically illustrated in (Figure 1). The mean Cd contents were compared with permitted level using student t-test. The results showed that there was a significant differences between cadmium contents of Rasht garlic and permitted value (P < 0.001), but there was no significant differences between those of Ramhor­moz (P = 0.388). Students t-test also showed significant differences between cadmium contents of the both cul­tivated areas (P = 0.037). Consequently it was concluded that Rasht garlic is contaminated.

Table 3 Analysis Results of Cd Contents of Garlic Cultivated in Ramhormoz and Rasht (Results are Mean of Two Measurements)

Figure 1 Graphical Comparison of Mean Concentration of Cadmium in Garlic Samples Collected from Ramhormoz and Rasht

According to primary investigations of the regions, farmers commonly use organo phosphorous fertilizers, especially simple super phosphate and triple super phosphate. Fertilizers contain high amount of Cadmium and Lead. But Pb bioavailability reduced via complexion of pb ions by anions such as hydroxide, carbonate, sulphate, sulfide, and especially phosphates. In comparison to the Ramhormoz’s farmers, the farmers of Rash use uncontrolled amount of these fertilizers, which is closely related to the higher amount of Cd detected in the collected garlic samples. The results show the mean concentration of cadmium in Gilan garlic is higher than those of Khozestan. It may be due to higher oregano phosphorous fertilizer consumption rate. Since daily garlic consumption as food and medicine is routine in this regions, an alternative fertilizer with less hazards is of an essence. Soil heavy metal contamination and irrigation water are other concerns, which should be prevented by heavy metal analyzes and cultivated area controls before growing any plant.

Authors gratefully acknowledge the financial support of research council of Jundishapur University of medical sciences for this Pharm D thesis under grant number u-86002.